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Image Search Results
Journal: PLoS ONE
Article Title: Purification of Bone Marrow Clonal Cells from Patients with Myelodysplastic Syndrome via IGF-IR
doi: 10.1371/journal.pone.0140372
Figure Lengend Snippet: (A) and (B) were isotype controls for the detection. (C) The expression of IGF-IR on CD134+ cells from 1 normal control was 4.3%. (D) Of these CD34+ IGF-IR+ cells, 75.3% expressed the erythroid lineage marker CD235a. (E) The expression of IGF-IR on bone marrow CD34 + cells was 82.9% in 1 MDS patient. (F) IGF-IR was expressed on the surface of 15.8% erythroid lineage cells from the same MDS patient. There was not significant expression of IGF-IR on the T lymphocytes (G) or B lymphocytes (H) from the same MDS patient. (I) The expression of IGF-IR of CD34 + cells from the 55 MDS patients was significantly higher than that of cells from the normal controls (54.0 vs. 4.5%; P <0.0001). (J) The expression of IGF-IR of CD34 + cells in the abnormal chromosome group was significantly higher than that in the normal chromosome group (72.0 vs. 50.4%; P = 0.019).
Article Snippet: CD34 + cells from 1×10 7 isolated mononuclear cells were sorted using a
Techniques: Expressing, Control, Marker
Journal: PLoS ONE
Article Title: Purification of Bone Marrow Clonal Cells from Patients with Myelodysplastic Syndrome via IGF-IR
doi: 10.1371/journal.pone.0140372
Figure Lengend Snippet: After CD34 + cells from 6 MDS patients were amplified in vitro , PPP was added at a concentration of 1 μM, and DMSO was used as a control. Cells at 0, 24, 48, 72, 96, and 168 h (7 d) after drug treatment were collected to calculate the mortality by Trypan Blue staining and smeared onto slides to detect the percentage of clonal cells using FISH. Over time, (A) the percentage of clonal cells progressively decreased. The P value resulted from the comparison between before PPP drug intervention (0 h) and 168 h (7 d) after intervention. The average percentage decreased from the initial 80.7 to 60.0%, and the difference was statistically significant. (B) The cell mortality increased over time and the difference from the control was statistically significant.
Article Snippet: CD34 + cells from 1×10 7 isolated mononuclear cells were sorted using a
Techniques: Amplification, In Vitro, Concentration Assay, Control, Staining, Comparison
Journal: NPJ Precision Oncology
Article Title: Single-cell analysis reveals CD34 + CD90 + endothelial cells promote tumor metastasis in gallbladder cancer
doi: 10.1038/s41698-025-01040-2
Figure Lengend Snippet: A Flow cytometry was used to detect CD34 + CD90 + ECs in 2 GBC tissues (PE: CD34, FITC: CD90). B Representative multiple immune staining images of CD34 and CD90 in GBC tissues. C Immune staining of CD34 and CD90 on GBC tissue microarray. D Analysis of the correlation between CD34 + or CD34 + CD90 + cell contents and patient survival prognosis and clinical characteristics. OS, overall survival time.
Article Snippet: Subsequently, FITC-conjugated CD90 antibody was added to the sorted CD34 + endothelial cells, and an
Techniques: Flow Cytometry, Staining, Microarray